Biodegradation of polycyclic aromatic hydrocarbons in crude oil. Although bacterial anaerobic degradation of monoaromatic compounds has been characterized in depth, the degradation of polycyclic aromatic hydrocarbons pahs such as naphthalene has only started. Degradation of polycyclic aromatic hydrocarbons by. Microbial degradation of hydrocarbons in the environment. Hl7b was chosen for further study because it oxidized several polycyclic aromatic hydrocarbons and aromatic heterocycles without an apparent. Das1 1 laboratory of environmental microbiology and ecology lenme, department of life science, national institute of technology, odisha, india. The demand for processed petroleum products and agricultural produce has exposed our environment to polycyclic aromatic hydrocarbons pahs contamination.
Anaerobic degradation of polycyclic aromatic hydrocarbons michael safinowski aromatic hydrocarbons belong to the most important ground water contaminants. Novosphingobium pentaromativorans us61, a marine bacterium isolated from muddy sediments of ulsan bay, republic of korea, was previously shown to be capable of degrading multiple polycyclic. Polycyclic aromatic hydrocarbons pahs is the common name for a class of several hundred different compounds, which typically contain two to seven condensed benzene rings. Many members of the sphingomonad genus isolated from different geological. Pseudomonas saccharophila p15 was isolated from soil contaminated with polycyclic aromatic hydrocarbons pah and previously was reported to degrade a variety of low and highmolecular weight pah. Metabolism of polycyclic aromatic hydrocarbons in the aquatic. Degradation of polycyclic aromatic hydrocarbons by actinomycetes. Ligninolytic fungi accomplish the partial degradation of numerous aromatic organopollutants. Polycyclic aromatic hydrocarbons pahs include a group of organic priority pollutants of critical environmental and public health concern due to their toxic, genotoxic, mutagenic andor. The metabolism of pahs by aerobic bacteria is usually initiated by dioxygenases, which incorporate both atoms of o2 into the pah to form one or more isomeric. A nonenzymic system containing cuso4 10 mmoll and hydrogen peroxide 100 mmoll was used for the degradation of three polycyclic aromatic hydrocarbons. Increasing the clo2 concentration increases the pah degradation rate.
Polycyclic aromatic hydrocarbons pahs polycyclic aromatic hydrocarbons pahs are a class of chemicals that occur naturally in coal, crude oil, and gasoline. Polycyclic aromatic hydrocarbons pahs are hydrocarbons composed of two or more benzene rings, and they are widely found in nature. The degradation of polycyclic aromatic hydrocarbons by a manganese peroxidase crude preparation of nematoloma frowardii was demonstrated for a mixture of eight different polycyclic aromatic hydrocarbons, and the five individual polycyclic aromatic hydrocarbons phenanthrene, anthracene, pyrene, fluoranthene, and benzoapyrene. Polycyclic aromatic hydrocarbons problem chemicals. Naphthalene, with its two rings, is considered the simplest polycyclic aromatic hydrocarbon by us epa and cdc for policy contexts. Interest in the biodegradation mechanisms and environmental fate of polycyclic aromatic hydrocarbons pahs is prompted by their ubiquitous distribution and their potentially deleterious.
Get a printable copy pdf file of the complete article 2. Pot experiment was carried out with 1 kg of soil in 7 litres perforated. Multiphase reactivity of polycyclic aromatic hydrocarbons. The degradation of pahs remains in a dynamic equilibrium. These sphingomonads such as sphingobium yanoikuyae strain b1, novosphingobium aromaticivorans strain f199, and sphingobium sp. Pahs are aromatic hydrocarbons with two or more fused benzene rings with natural as well as anthropogenic sources. Their ability to degrade polycyclic aromatic hydrocarbons pahs is particularly interesting because eukaryotes were previously considered to be unable to cleave fusedring aromatics. Whether in mouse pads, toys, or bathing shoes polycyclic aromatic hydrocarbons pahs are ubiquitous.
Polycyclic aromatic hydrocarbons pahs are potent envi ronmental pollutants that comprise fused aromatic rings. Polycyclic aromatic hydrocarbons degradation techniques. Mechanisms for polycyclic aromatic hydrocarbon degradation by. Pahs constitute a large and diverse class of organic compounds and are generally. Transformation and degradation of polycyclic aromatic. Pdf fungal degradation of polycyclic aromatic hydrocarbons. Pdf current state of knowledge in microbial degradation of. Most frequently, nonbranded products at affordable prices get negative attention in this respect, but products from renowned companies may contain pahs as well.
Abstract polycyclic aromatic hydrocarbons pahs are pollutants that occur in mangrove sediments. Salicylate stimulates the degradation of highmolecular. Degradation of polycyclic aromatic hydrocarbons dissolved in tween 80 surfactant solutions by sphingomonas paucimobilis epa 505 dianne j. Degradation of polycyclic aromatic hydrocarbons and aromatic. Pdf degradation of polycyclic aromatic hydrocarbons by the. Peng et al degradation of polycyclic aromatic hydrocarbons. The environmental protection agency epa has identified 1,408 hazardous waste sites as the most serious in the nation.
Recent studies suggest possible involvement of plasmid in hmwpah degradation than was previously thought. This study investigated the biodegradation of highmolecularweight polycyclic aromatic hydrocarbons pahs in liquid media and soil by bacteria stenotrophomonas maltophilia vun 10,010. Biodegradation of polycyclic aromatic hydrocarbons sciencedirect. Sr wild, ml berrow, kc jonesthe persistence of polynuclear aromatic hydrocarbons pahs in sewage sludge amended agricultural soils. Pseudomonas saccharophila p15 was isolated from soil contaminated with polycyclic aromatic hydrocarbons pah and previously was reported to degrade a variety of low and highmolecular. The disappearance of the original pah was determined for the freshly spiked soils, for soils incubated for up to 287 d with their indigenous microflora, and for autoclaved, unsterile and pasteurized soils inoculated with. This study investigated the biodegradation of highmolecularweight polycyclic aromatic hydrocarbons pahs in liquid media and soil by bacteria stenotrophomonas maltophilia vun 10,010 and bacterial consortium vun 10,009 and a fungus penicillium janthinellum vuo 10,201 that were isolated from separate creosote and manufacturedgas plantcontaminated soils. Biodegradation of polycyclic aromatic hydrocarbon in petroleum oil contaminating. The effect of polycyclic aromatic hydrocarbons on the. Degradation of polycyclic aromatic hydrocarbons by moderately.
Oxidative degradation of polycyclic aromatic hydrocarbons in contaminated industrial soil using chlorine dioxide. Biodegradation aspects of polycyclic aromatic hydrocarbons pahs. Pdf degradation of polycyclic aromatic hydrocarbons by. Pdf current state of knowledge in microbial degradation. Current state of knowledge in microbial degradation of polycyclic aromatic hydrocarbons pahs. Anaerobic degradation of polycyclic aromatic hydrocarbons. Man slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Pah biodegradation, planomicrobium, rhodococcus, phenanthrene, pyrene. Their ability to degrade polycyclic aromatic hydrocarbons pahs is particularly interesting because. Qualitative tests with and without peptone as a nitrogen source implied enzymatic. Aspects of sampling, sample preparations such as extraction of pahs are discussed.
Novosphingobium pentaromativorans us61, a marine bacterium isolated from muddy sediments of ulsan bay, republic of korea, was previously shown to be capable of degrading multiple polycyclic aromatic hydrocarbons pahs. This statement was prepared to give you information about polycyclic aromatic hydrocarbons pahs and to emphasize the human health effects that may result from exposure to them. Influential factors, implications and recommendations author links open overlay panel gustav gbeddy ashantha goonetilleke godwin a. Qualitative tests with and without peptone as a nitrogen source implied enzymatic degradation. Accelerated parathion degradation in soil inoculated with acclimated bacteria under field conditions. The degradation of polycyclic aromatic hydrocarbons by a manganese peroxidase crude preparation of nematoloma frowardii was demonstrated for a mixture of eight different polycyclic aromatic. Current state of knowledge in microbial degradation of. In screening indigenous soil filamentous fungi for polycyclic aromatic hydrocarbons pahs degradation, an isolate of the fusarium solani was found to incorporate benzoapyrene bap into fungal.
Polycyclic aromatic hydrocarbons degradation by marine. Apr 24, 2016 polycyclic aromatic hydrocarbons pahs are widely distributed and relocated in the environment as a result of the incomplete combustion of organic matter. Most of the pahs with low vapour pressure in the air are adsorbed on particles. By definition, polycyclic aromatic hydrocarbons have multiple cycles, precluding benzene from being considered a pah. Strain p15 grows on phenanthrene by a known pathway in which salicylate is an intermediate.
Microbial degradation of polycyclic aromatic hydrocarbons in. Polycyclic aromatic hydrocarbons pahs are widely distributed and relocated in the environment as a result of the incomplete combustion of organic matter. In this study the potential application of phenoloxidizing and nitrifying bacteria as priming. Preincubation with phenanthrene and downstream intermediates through salicylate stimulated pah dioxygenase.
Bacterial degradation and bioremediation of polycyclic. However, typical oxidation systems are limited by the acidic environment needed for fenton reactions, cost and soil effects of activated persulfate, and large pores required for ozone or selected oxidation by permanganate. Mechanisms for polycyclic aromatic hydrocarbon degradation. The potential of some actinomycetes to degrade polycyclic aromatic hydrocarbons pah and the effect of cosubstrates, plants and other additives on their degradation and bioavailability was studied. Mechanisms of the aqueous photodegradation of polycyclic. Although studies related to the microbial degradation of pahs have been carried out in the last decades, little is known about degradation of these environmental pollutants by fungi from marine origin. Metabolism of polycyclic aromatic hydrocarbons in the aquatic environment crc press book this book shows how the biological transport, bioaccumulation, disposition, and toxicity of polycyclic aromatic. Other authors consider pahs to start with the tricyclic species phenanthrene and anthracene. This paper describes the degradation of naphthalene, phenanthrene, anthracene, fluoranthene, and pyrene in soil and soilcompost mixtures. Bacteria play an important role in the removal of polycyclic aromatic hydrocarbons pahs from polluted environments. However, little is known regarding the degradation mechanisms for multiple pahs by cycloclasticus. Transformation and degradation of polycyclic aromatic hydrocarbons pahs in urban road.
Although bacterial anaerobic degradation of mono aromatic compounds has been characterized in depth, the degradation of polycyclic aromatic hydrocarbons pahs such as naphthalene has only started. Compost addition facilitated the degradation of 500 mg naphthalenekg soil and 100 mgkg each of other polycyclic aromatic hydrocarbons pah within 25 days in soil systems with water contents below the waterholding capacity. We reveal for the first time that polycyclic aromatic hydrocarbon pah degradation is a shared phenotype among members of this genus, indicating that it could be used as a taxonomic marker for. Polycyclic aromatic hydrocarbons pahs are organic compounds that consist of either at least two condensed benzene. Their presence in these media creates problems because consuming products obtained from these sources could be deleterious to human health since several of these. Pahs stick to solid sediments and are ubiquitous including soil, water and air. In order to gain insight into the characteristics of pahs degradation, a proteome analysis of n. Transformation and degradation of polycyclic aromatic hydrocarbons pahs in urban road surfaces. Biodegradation of highmolecularweight polycyclic aromatic. The unique aromatic catabolic genes in sphingomonads. Degradation and mineralization of highmolecularweight.
Microbial degradation of polycyclic aromatic hydrocarbons. Degrader biomass and pah degradation were invariably negatively correlated. The increased awareness of their various adverse effects on ecosystem and human health has led to a dramatic increase in. The microbial degradation of pahs such as naphthalene. Aug 31, 2016 polycyclic aromatic hydrocarbons pahs include a group of organic priority pollutants of critical environmental and public health concern due to their toxic, genotoxic, mutagenic andor carcinogenic properties and their ubiquitous occurrence as well as recalcitrance. They are found almost everywhere in the environment and are typically formed during incomplete burning of. Pdf degradation of polycyclic aromatic hydrocarbons. Enrichment cultures were established with the aromatic fraction of a crude oil and screened for aromaticdegrading pseudomonads, using a sprayed plate technique. Pdf effect of temperature on the formation and degradation. Interest in the biodegradation mechanisms and environmental fate of pahs is prompted by their ubiquitous distribution in the environment and their deleterious effects on human health see table 1 for cancer classi. Polycyclic aromatic hydrocarbon pah degradation pathways of. Biodegradation aspects of polycyclic aromatic hydrocarbons. Degradation of polycyclic aromatic hydrocarbons and. Degradation of polycyclic aromatic hydrocarbons with three.
Biodegradation of polycyclic aromatic hydrocarbons in. Polycyclic aromatic hydrocarbon pah degradation pathways. Polycyclic aromatic hydrocarbons pahs are prone to postemission transformation and degradation to yield transformed pah products tpps that are potentially more hazardous than parent pahs. In marine environments, cycloclasticus is one of the most prevalent pah. We reveal for the first time that polycyclic aromatic hydrocarbon pah degradation is a shared phenotype among members of this genus, indicating that it could be used as a taxonomic marker for this genus. Degradation of polycyclic aromatic hydrocarbons by manganese. The removal of pahs was significantly decreased at higher soil ph values ph 7. Pahs are aromatic hydrocarbons with two or more fused benzene rings with natural as well as anthropogenic.
Biodegradation of polycyclic aromatic hydrocarbons by. The information acquired so far on the anaerobic degradation of polycyclic aromatic hydrocarbons shows definitely that at least naphthalene and other tworing pahs can be used as the sole source of carbon and energy under sulfate, iron, and nitratereducing conditions. They have a relatively low solubility in water, but are highly lipophilic. Polycyclic aromatic hydrocarbons pahs are among the most prominent toxic compounds in the air. The unique aromatic catabolic genes in sphingomonads degrading polycyclic aromatic hydrocarbons pahs onruthai. Metabolism of polycyclic aromatic hydrocarbons in the aquatic environment crc press book this book shows how the biological transport, bioaccumulation, disposition, and toxicity of polycyclic aromatic hydrocarbons pah in the aquatic environment are influenced by the ability or inability of organisms to metabolize these environmental pollutants. Simultaneous analysis of the photocatalytic degradation of polycyclic aromatic hydrocarbons using threedimensional excitationemission matrix fluorescence and parallel factor analysis. In marine environments, cycloclasticus is one of the most prevalent pahdegrading bacterial genera. Pdf polycyclic aromatic hydrocarbons degradation techniques.
Anaerobic microbial degradation of polycyclic aromatic. Pyrene and benzoapyrene bap are high molecular weight polycyclic aromatic hydrocarbons pahs recalcitrant to microbial attack. Pdf thirteen deuteromycete ligninolytic fungal strains were grown in media containing polycyclic aromatic hydrocarbons pahs, for 6 and 10 days. Interest in the biodegradation mechanisms and environmental fate of polycyclic aromatic hydrocarbons pahs is prompted by their ubiquitous distribution and their potentially deleterious effects on human health. Das1 1 laboratory of environmental microbiology and. Bacterial degradation and bioremediation of polycyclic aromatic hydrocarbons article pdf available in polish journal of environmental studies 121. Cometabolic biodegradation processes are potentially useful for the bioremediation of hazardous waste sites.
Effects of cooccurring aromatic hydrocarbons on degradation of individual polycyclic aromatic hydrocarbons in marine sediment slurries. Degradation of polycyclic aromatic hydrocarbons with three to. Highlightsthe study investigates the removal of pahs in contaminated soil using clo2. Oxidative degradation of polycyclic aromatic hydrocarbons. In situ chemical oxidation can be used to remediate soils contaminated with polycyclic aromatic hydrocarbons pahs. Heterogeneous reactions involving o3 can change the toxicity of pahs, but the. Pdf biodegradation of polycyclic aromatic hydrocarbons by soil. Enrichment cultures were established with the aromatic fraction of a crude oil and screened for aromatic degrading pseudomonads, using a sprayed plate technique.
The information acquired so far on the anaerobic degradation of polycyclic aromatic hydrocarbons shows definitely that at least naphthalene and other tworing pahs can be used as the. Effect of temperature on the formation and degradation of polycyclic aromatic hydrocarbons. Metabolism of polycyclic aromatic hydrocarbons in the. Degradation of polycyclic aromatic hydrocarbons mafiadoc. The recent literature describing polycyclic aromatic hydrocarbons pahs in air, water, soil and sediment, waste sludge.
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